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ACTH Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human pituitary adenoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ACTH ) Monoclonal Antibody, Unconjugated (bsm-34147M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human pituitary adenoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ACTH ) Monoclonal Antibody, Unconjugated (bsm-34147M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Bioss unconjugated antibody vial

Applications

  • IHC-F
  • IF(IHC-P)
  • IHC-P

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bsm-34147m
Product Name ACTH Monoclonal Antibody
Applications
IHC-F IF(IHC-P) IHC-P
Reactivity Human
Predicted Reactivity Mouse, Rat
Specifications
Conjugation Unconjugated
Host Mouse
Source KLH conjugated synthetic peptide derived from human ACTH
Clonality Monoclonal
Clone # 7D13
Isotype IgG1/kappa
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 5443
Swiss Prot P01189
Subcellular location Secreted
Synonyms Adrenocorticotropic hormone; Adrenocorticotropin; Adrenocorticotropin Hormone; Alpha Melanocyte Stimulating Hormone; Beta Lipotropin; Beta Melanocyte Stimulating Hormone; CLIP; Corticotropin; Corticotropin Like Intermediary Peptide; Corticotropin lipotropin precursor; Lipotropin Beta; Lipotropin Gamma; LPH; Melanotropin Alpha; Met Enkephalin; MSH; NPP; POC; POMC; Pro opiomelanocortin; Proopiomelanocortin; COLI_HUMAN.
Application Dilution
IHC-F 1:100-500
IF(IHC-P) IFIHC-P1:100-500
IHC-P IHC-P1:100-500

Frequently asked questions

Which applications is ACTH Monoclonal Antibody (bsm-34147M) validated for, and at what dilution?

bsm-34147M is validated for IHC-F(IHC-F) (1:100-500); IF(IHC-P)(IF(IHC-P)) (1:100-500); IHC-P(IHC-P) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ACTH Monoclonal Antibody (bsm-34147M) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.

How should bsm-34147M be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ACTH Monoclonal Antibody (bsm-34147M)?

KLH conjugated synthetic peptide derived from human ACTH

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