Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch
Help

TTF1/NKX2-1 Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (TTF1\/NKX2-1 ) Monoclonal Antibody, Unconjugated (bsm-34158M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (TTF1\/NKX2-1 ) Monoclonal Antibody, Unconjugated (bsm-34158M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (TTF1\/NKX2-1 ) Monoclonal Antibody, Unconjugated (bsm-34158M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (TTF1\/NKX2-1 ) Monoclonal Antibody, Unconjugated (bsm-34158M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (TTF1\/NKX2-1 ) Monoclonal Antibody, Unconjugated (bsm-34158M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (TTF1\/NKX2-1 ) Monoclonal Antibody, Unconjugated (bsm-34158M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (TTF1\/NKX2-1 ) Monoclonal Antibody, Unconjugated (bsm-34158M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (TTF1\/NKX2-1 ) Monoclonal Antibody, Unconjugated (bsm-34158M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Bioss unconjugated antibody vial

Applications

  • IHC-F
  • IF(IHC-P)
  • IHC-P

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-34158m
Product Name TTF1/NKX2-1 Monoclonal Antibody
Applications
IHC-F IF(IHC-P) IHC-P
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Mouse
Source KLH conjugated synthetic peptide derived from human TTF-1
Clonality Monoclonal
Clone # 5B12
Isotype IgG1, Kappa
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer PBS, pH7.2, 0.02% Porcolin 300, containing stabilizing protein.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 7080
Swiss Prot P43699
Subcellular location Nucleus
Synonyms NKX21_HUMAN; Homeobox protein Nkx-2.1; Homeobox protein NK-2 homolog A; Thyroid nuclear factor 1; Thyroid transcription factor 1(TTF-1); Thyroid-specific enhancer-binding protein(T/EBP); NKX2A; TITF1; TTF-1; NK2 homeobox 1; BCH; BHC; NK-2; TEBP; NMTC1; T/EBP;
Application Dilution
IHC-F 1:100-500
IF(IHC-P) IFIHC-P1:100-500
IHC-P IHC-P1:100-500

Frequently asked questions

Which applications is TTF1/NKX2-1 Monoclonal Antibody (bsm-34158M) validated for, and at what dilution?

bsm-34158M is validated for IHC-F(IHC-F) (1:100-500); IF(IHC-P)(IF(IHC-P)) (1:100-500); IHC-P(IHC-P) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TTF1/NKX2-1 Monoclonal Antibody (bsm-34158M) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-34158M be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: PBS, pH7.2, 0.02% Porcolin 300, containing stabilizing protein.

What immunogen was used to raise TTF1/NKX2-1 Monoclonal Antibody (bsm-34158M)?

KLH conjugated synthetic peptide derived from human TTF-1

Search our catalog of hundreds of thousands of products