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Placental alkaline phosphatase (PLAP) Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Placental alkaline phosphatase (PLAP)) Monoclonal Antibody, Unconjugated (bsm-34165M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024)instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Placental alkaline phosphatase (PLAP)) Monoclonal Antibody, Unconjugated (bsm-34165M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024)instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Placental alkaline phosphatase (PLAP)) Monoclonal Antibody, Unconjugated (bsm-34165M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024)instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Placental alkaline phosphatase (PLAP)) Monoclonal Antibody, Unconjugated (bsm-34165M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024)instructionsand DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with Placental alkaline phosphatase (PLAP) monoclonal antibody, unconjugated (bsm-34165M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Placental alkaline phosphatase (PLAP) monoclonal antibody, unconjugated (bsm-34165M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Bioss unconjugated antibody vial

Applications

  • WB

Reactivity

  • Human
Overview
Catalog # bsm-34165m-100uL
Product Name Placental alkaline phosphatase (PLAP) Monoclonal Antibody
Applications
WB
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source KLH conjugated synthetic peptide derived from human Placental alkaline phosphatase
Clonality Monoclonal
Clone # 5C9
Isotype IgG1, Kappa
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Proclin300.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 250
Swiss Prot P05187
Subcellular location Cell membrane
Synonyms Alkaline phosphatase; Alkaline phosphatase placental; Alkaline phosphatase placental type; Alkaline phosphatase Regan isozyme; ALP; Alp1; ALPP; FLJ61142; Germ-cell alkaline phosphatase; nagao isozyme; OTTHUMP00000164354; PALP; Placental alkaline phosphatase 1; placental heat-stable alkaline phosphatase; placental type; PLAP; PLAP-1; PLAP1; PPB1_HUMAN.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is Placental alkaline phosphatase (PLAP) Monoclonal Antibody (bsm-34165M) validated for, and at what dilution?

bsm-34165M is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Placental alkaline phosphatase (PLAP) Monoclonal Antibody (bsm-34165M) react with?

Tested reactivity: Human.

How should bsm-34165M be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Proclin300.

What immunogen was used to raise Placental alkaline phosphatase (PLAP) Monoclonal Antibody (bsm-34165M)?

KLH conjugated synthetic peptide derived from human Placental alkaline phosphatase

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