LCN2, AbBy Fluor-555 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-41179M-BF555 |
| Product Name | LCN2, AbBy Fluor-555 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Mouse |
| Source | Recombinant human LCN2 |
| Clonality | Monoclonal |
| Clone # | #REF! |
| Isotype | IgG1 |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3934 |
| Swiss Prot | P80188 |
| Subcellular location | Secreted |
| Synonyms | Lipocalin 2; Neutrophil Gelatinase associated Lipocalin; 25 kDa alpha 2 microglobulin related subunit of MMP9; Alpha 2 microglobulin related protein; HGNC:6526; HNL; Lcn 2; LCN-2; Neutrophil gelatinase associated lipocalin precursor; NGAL; Oncogene 24p3; P25; SV40 induced 24P3 protein. 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Migration stimulating factor inhibitor; MSFI; Neutrophil gelatinase associated lipocalin precursor; Neutrophil gelatinase-associated lipocalin; NGAL_HUMAN; Oncogenic lipocalin 24p3; Siderocalin; siderocalin LCN2; SV40 induced 24P3 protein. |
| Background | Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is LCN2, AbBy Fluor-555 Conjugated (bsm-41179M-BF555) validated for, and at what dilution?
bsm-41179M-BF555 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LCN2, AbBy Fluor-555 Conjugated (bsm-41179M-BF555) react with?
Tested reactivity: Human.
How should bsm-41179M-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LCN2, AbBy Fluor-555 Conjugated (bsm-41179M-BF555)?
Recombinant human LCN2
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bsm-41179M-BF555)?
AbBy Fluor-555 on bsm-41179M-BF555: excitation / emission 553nm/568nm.