CD34 Monoclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-41197M-Cy3-100uL |
| Product Name | CD34 Monoclonal Antibody, Cy3 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Mouse |
| Source | Recombinant human CD34 |
| Clonality | Monoclonal |
| Clone # | 5B4 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 947 |
| Swiss Prot | P28906 |
| Subcellular location | Cytomembrane |
| Synonyms | CD34 antigen; CD34 molecule; Cluster designation 34; Hematopoietic progenitor cell antigen CD34; HPCA1; CD34_HUMAN; Hematopoietic Stem Cell Marker; Endothelial Markers; Tumor biomarkers. |
| Background | Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is CD34 Monoclonal Antibody, Cy3 Conjugated (bsm-41197M-Cy3) validated for, and at what dilution?
bsm-41197M-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD34 Monoclonal Antibody, Cy3 Conjugated (bsm-41197M-Cy3) react with?
Tested reactivity: Human.
How should bsm-41197M-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD34 Monoclonal Antibody, Cy3 Conjugated (bsm-41197M-Cy3)?
Recombinant human CD34
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-41197M-Cy3)?
Cy3 on bsm-41197M-Cy3: excitation / emission 512,550nm/570,615nm.