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ZO-1 Monoclonal Antibody, Cy5.5 Conjugated

Applications

  • WB

Reactivity

  • Human
Overview
Catalog # bsm-41327m-cy55-100ul
Product Name ZO-1 Monoclonal Antibody, Cy5.5 Conjugated
Applications
WB
Reactivity Human
Specifications
Conjugation Cy5.5
Host Mouse
Source Recombinant human ZO-1 protein
Clonality Monoclonal
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7082
Swiss Prot Q07157
Subcellular location Cytoplasm, Cell membrane
Synonyms ZO1_HUMAN; Tight junction protein ZO-1; TJP1; ZO1; Tight junction protein 1; Zona occludens protein 1; Zonula occludens protein 1; ZO1 tight junction protein;
Background The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.
Application Dilution
WB 1:1000-5000

Frequently asked questions

Which applications is ZO-1 Monoclonal Antibody, Cy5.5 Conjugated (BSM-41327M-CY5-5) validated for, and at what dilution?

BSM-41327M-CY5-5 is validated for WB(WB) (1:1000-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ZO-1 Monoclonal Antibody, Cy5.5 Conjugated (BSM-41327M-CY5-5) react with?

Tested reactivity: Human.

How should BSM-41327M-CY5-5 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ZO-1 Monoclonal Antibody, Cy5.5 Conjugated (BSM-41327M-CY5-5)?

Recombinant human ZO-1 protein

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BSM-41327M-CY5-5)?

Cy5.5 on BSM-41327M-CY5-5: excitation / emission 675nm/694nm.