Beclin 1 Recombinant Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-41365r-apc-cy7-100uL |
| Product Name | Beclin 1 Recombinant Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | Recombinant human Beclin 1 protein |
| Immunogen Range | 141-450/450 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8678 |
| Swiss Prot | Q14457 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Beclin-1; ATG6; beclin1; VPS30; Beclin 1 like protein 1; 4921513J16Rik; 5430417M23Rik; ATG6; BECLIN 1; BECN1; MGC6843; BECN1_HUMAN. |
| Background | Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl 2 binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 52kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons. |
| Application Dilution | |
| WB | 1:200-1000 |
| IF(IHC-P) | IFIHC-P1:50-200 |
Frequently asked questions
Which applications is Beclin 1 Recombinant Antibody, APC-Cy7 Conjugated (bsm-41365R-APC-Cy7) validated for, and at what dilution?
bsm-41365R-APC-Cy7 is validated for WB(WB) (1:200-1000); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Beclin 1 Recombinant Antibody, APC-Cy7 Conjugated (bsm-41365R-APC-Cy7) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.
How should bsm-41365R-APC-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Beclin 1 Recombinant Antibody, APC-Cy7 Conjugated (bsm-41365R-APC-Cy7)?
Recombinant human Beclin 1 protein Immunogen range: 141-450/450.
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bsm-41365R-APC-Cy7)?
APC-Cy7 on bsm-41365R-APC-Cy7: excitation / emission 650nm/780nm.