SARS-CoV-2 (2019-nCoV) Nucleocapsid (7E1B) Monoclonal Antibody
Applications
| Overview | |
| Catalog # | bsm-41414m-100uL |
| Product Name | SARS-CoV-2 (2019-nCoV) Nucleocapsid (7E1B) Monoclonal Antibody |
| Applications |
WB
ELISA
|
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone # | 7E1B |
| Isotype | IgG2b |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | 0.01M PBS (pH7.4) with 0.02% Proclin300. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Swiss Prot | P0DTC9 |
| Synonyms | SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2]; novel coronavirus N Protein; novel coronavirus Nucleocapsid Protein; 2019-nCoV Nucleoprotein; 2019-nCoV N; 2019nCoV N; 2019-nCoV N Protein; 2019 peak N Protein; 2019-nCoV nucleocapsid protein. |
| Background | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| Application Dilution | |
| WB | 1:500-1000 |
| ELISA | 1:100-500 |
Frequently asked questions
Which applications is SARS-CoV-2 (2019-nCoV) Nucleocapsid (7E1B) Monoclonal Antibody (bsm-41414M) validated for, and at what dilution?
bsm-41414M is validated for WB (1:500-1000); ELISA (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bsm-41414M be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M PBS (pH7.4) with 0.02% Proclin300.