SARS-CoV-2 (2019-nCoV) Nucleocapsid (9D2), WT & Ormicron Recognized Monoclonal Antibody

$Lane 1: SARS-CoV-2 N protein(WT) at 10ng; Lane 2: SARS-CoV-2 N protein(Q9H, P67S, P80R, P151L, S183Y) at 2ng; Lane 3: SARS-CoV-2 N protein (D3L, P13T, D103Y, D128Y,H145Y, R203K, G204R, T205I, S235F) at 2ng; Lane 4: SARS-CoV-2 N protein (Del204 & Del215) at 2ng; Lane 5: SARS-CoV-2 N protein (R203M & D377Y) at 2ng probed with anti-SARS-CoV-2 N protein monoclonal antibody, Unconjugated (bsm-41481M) at 1:1000 dilution and 4\u2103 overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60min at 37 \u2103.$

Lane 1: SARS-CoV-2 N protein(WT) at 10ng; Lane 2: SARS-CoV-2 N protein(Q9H, P67S, P80R, P151L, S183Y) at 2ng; Lane 3: SARS-CoV-2 N protein (D3L, P13T, D103Y, D128Y,H145Y, R203K, G204R, T205I, S235F) at 2ng; Lane 4: SARS-CoV-2 N protein (Del204 & Del215) at 2ng; Lane 5: SARS-CoV-2 N protein (R203M & D377Y) at 2ng probed with anti-SARS-CoV-2 N protein monoclonal antibody, Unconjugated (bsm-41481M) at 1:1000 dilution and 4\u2103 overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60min at 37 \u2103.

$Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 NP (bs-41417P) at 2\u03bcg\/mL (100\u03bcL\/well) can bind anti-SARS-CoV-2 NP monoclonal antibody, the EC50 for this effect is 3.6-7.8ng\/ml.$

Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 NP (bs-41417P) at 2\u03bcg\/mL (100\u03bcL\/well) can bind anti-SARS-CoV-2 NP monoclonal antibody, the EC50 for this effect is 3.6-7.8ng\/ml.

Applications

ELISA
LFA

Reactivity

Mouse
SARS-CoV-2

Overview
Catalog #	bsm-41481M
Product Name	SARS-CoV-2 (2019-nCoV) Nucleocapsid (9D2), WT & Ormicron Recognized Monoclonal Antibody
Applications	ELISA, LFA
Reactivity	Mouse, SARS-CoV-2
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant SARS-CoV-2 Nucleocapsid Protein (full length)
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG1
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	43740575
Swiss Prot	P0DTC9
Background	Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Application Dilution
ELISA	1:500-1000
LFA	N/A