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GFAP Monoclonal Antibody

25 ug total protein per lane of various lysates (see on figure) probed with GFAP monoclonal antibody, unconjugated (bsm-41823M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with GFAP monoclonal antibody, unconjugated (bsm-41823M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-41823M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-41823M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-41823M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-41823M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF(IHC-P)

Reactivity

  • Human
Overview
Catalog # bsm-41823m
Product Name GFAP Monoclonal Antibody
Applications
WB IHC-P IHC-F IF(IHC-P)
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Recombinant human GFAP protein
Immunogen Range 1-390/432
Clonality Monoclonal
Clone # 12C12
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location Cytoplasm
Synonyms Astrocyte Marker; FLJ45472; Glial Fibrillary Acidic Protein; Intermediate filament protein; GFAP_HUMAN.
Background GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Application Dilution
WB 1:1000-5000
IHC-P IHC-P1:200-800
IHC-F IHC-F1:200-800
IF(IHC-P) IFIHC-P1:200-800

Frequently asked questions

Which applications is GFAP Monoclonal Antibody (bsm-41823M) validated for, and at what dilution?

bsm-41823M is validated for WB(WB) (1:1000-5000); IHC-P(IHC-P) (1:200-800); IHC-F(IHC-F) (1:200-800); IF(IHC-P)(IF(IHC-P)) (1:200-800). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GFAP Monoclonal Antibody (bsm-41823M) react with?

Tested reactivity: Human.

How should bsm-41823M be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GFAP Monoclonal Antibody (bsm-41823M)?

Recombinant human GFAP protein Immunogen range: 1-390/432.

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