NF-L Recombinant Antibody

$The SH-SY5Y (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-NF-L antibody (bsm-41836R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The SH-SY5Y (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-NF-L antibody (bsm-41836R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.

$4% Paraformaldehyde-fixed SH-SY5Y (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NF-L) monoclonal Antibody, unconjugated (bsm-41836R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

4% Paraformaldehyde-fixed SH-SY5Y (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NF-L) monoclonal Antibody, unconjugated (bsm-41836R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

Flow-Cyt
ICC/IF

Reactivity

Human

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bsm-41836r-100ul
Product Name	NF-L Recombinant Antibody
Applications	Flow-Cyt, ICC/IF
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human NF-L protein
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4747
Swiss Prot	P07196
Subcellular location	Cytoplasm
Synonyms	CMT1F; CMT2E; CMTDIG; NF-L; NF68; NFL; PPP1R110; NFL_HUMAN; NEFL; 68 kDa neurofilament protein; Neurofilament triplet L protein; NFL_MOUSE; NFL_RAT;
Application Dilution
Flow-Cyt	=1μg/Test
ICC/IF	ICC/IF=1:50-200