GFAP Recombinant Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-42001R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-42001R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-42001R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-42001R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-42001R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-42001R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-42001R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-42001R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-42001R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-42001R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-42001R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-42001R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-42001R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Monoclonal Antibody, Unconjugated(bsm-42001R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Polyclonal Antibody, Unconjugated (bsm-42001R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-FITC), DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Polyclonal Antibody, Unconjugated (bsm-42001R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-FITC), DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Polyclonal Antibody, Unconjugated (bsm-42001R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-FITC), DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Polyclonal Antibody, Unconjugated (bsm-42001R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-FITC), DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Polyclonal Antibody, Unconjugated (bsm-42001R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-FITC), DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GFAP Polyclonal Antibody, Unconjugated (bsm-42001R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-FITC), DAPI (blue, C02-04002) was used to stain the cell nuclei.

$25 ug total protein per lane of various lysates (see on figure) probed with GFAP monoclonal antibody, unconjugated (bsm-42001R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with GFAP monoclonal antibody, unconjugated (bsm-42001R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GFAP Monoclonal Antibody, Unconjugated (bsm-42001R) at 1:500 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose Red, bs-0295G-BF647), DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GFAP Monoclonal Antibody, Unconjugated (bsm-42001R) at 1:500 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose Red, bs-0295G-BF647), DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GFAP Monoclonal Antibody, Unconjugated (bsm-42001R) at 1:500 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose Red, bs-0295G-BF647), DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GFAP Monoclonal Antibody, Unconjugated (bsm-42001R) at 1:500 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose Red, bs-0295G-BF647), DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GFAP Monoclonal Antibody, Unconjugated (bsm-42001R) at 1:500 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose Red, bs-0295G-BF647), DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GFAP Monoclonal Antibody, Unconjugated (bsm-42001R) at 1:500 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose Red, bs-0295G-BF647), DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GFAP Monoclonal Antibody, Unconjugated (bsm-42001R) at 1:500 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (orange, bs-0295G-Cy3), DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GFAP Monoclonal Antibody, Unconjugated (bsm-42001R) at 1:500 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (orange, bs-0295G-Cy3), DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GFAP Monoclonal Antibody, Unconjugated (bsm-42001R) at 1:500 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (orange, bs-0295G-Cy3), DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GFAP Monoclonal Antibody, Unconjugated (bsm-42001R) at 1:500 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (orange, bs-0295G-Cy3), DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GFAP Monoclonal Antibody, Unconjugated (bsm-42001R) at 1:500 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (orange, bs-0295G-Cy3), DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GFAP Monoclonal Antibody, Unconjugated (bsm-42001R) at 1:500 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (orange, bs-0295G-Cy3), DAPI (blue, C02-04002) was used to stain the cell nuclei.

$The U-251 MG (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-GFAP antibody (bsm-42001R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n$

The U-251 MG (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-GFAP antibody (bsm-42001R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum.\r\nMerged staining of anti-GFAP (bsm-42001R; 1:200; pink) anti-AIF1\/Iba1 (bsm-54132R; 1:200; red) anti-NeuN (bsm-52268R; 1:200; green) anti-MBP (bsm-33932M; 1:200; yellow) and anti-Olig2 (bsm-61115R; 1:200; light blue) .\r\nDAPI (dark blue) was used as a nuclear counter stain.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum.\r\nMerged staining of anti-GFAP (bsm-42001R; 1:200; pink) anti-AIF1\/Iba1 (bsm-54132R; 1:200; red) anti-NeuN (bsm-52268R; 1:200; green) anti-MBP (bsm-33932M; 1:200; yellow) and anti-Olig2 (bsm-61115R; 1:200; light blue) .\r\nDAPI (dark blue) was used as a nuclear counter stain.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum.\r\nMerged staining of anti-GFAP (bsm-42001R; 1:200; pink) anti-AIF1\/Iba1 (bsm-54132R; 1:200; red) anti-NeuN (bsm-52268R; 1:200; green) anti-MBP (bsm-33932M; 1:200; yellow) and anti-Olig2 (bsm-61115R; 1:200; light blue) .\r\nDAPI (dark blue) was used as a nuclear counter stain.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum.\r\nMerged staining of anti-GFAP (bsm-42001R; 1:200; pink) anti-AIF1\/Iba1 (bsm-54132R; 1:200; red) anti-NeuN (bsm-52268R; 1:200; green) anti-MBP (bsm-33932M; 1:200; yellow) and anti-Olig2 (bsm-61115R; 1:200; light blue) .\r\nDAPI (dark blue) was used as a nuclear counter stain.

$Paraformaldehyde-fixed, paraffin embedded Human Cerebrum.\r\nMerged staining of anti-GFAP (bsm-42001R; 1:200; pink) anti-AIF1\/Iba1 (bsm-54132R; 1:200; red) anti-NeuN (bsm-52268R; 1:200; green) anti-MBP (bsm-33932M; 1:200; yellow) and anti-Olig2 (bsm-61115R; 1:200; light blue) .\r\nDAPI (dark blue) was used as a nuclear counter stain.$

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum.\r\nMerged staining of anti-GFAP (bsm-42001R; 1:200; pink) anti-AIF1\/Iba1 (bsm-54132R; 1:200; red) anti-NeuN (bsm-52268R; 1:200; green) anti-MBP (bsm-33932M; 1:200; yellow) and anti-Olig2 (bsm-61115R; 1:200; light blue) .\r\nDAPI (dark blue) was used as a nuclear counter stain.

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min.\r\nThe first round ,antibody incubation with S100B Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488). The second round , antibody incubation with GFAP Monoclonal Antibody, Unconjugated (bsm-42001R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red, bs-0295G-BF594). DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min.\r\nThe first round ,antibody incubation with S100B Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488). The second round , antibody incubation with GFAP Monoclonal Antibody, Unconjugated (bsm-42001R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red, bs-0295G-BF594). DAPI (blue, C02-04002) was used to stain the cell nuclei.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min.\r\nThe first round ,antibody incubation with S100B Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488). The second round , antibody incubation with GFAP Monoclonal Antibody, Unconjugated (bsm-42001R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red, bs-0295G-BF594). DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min.\r\nThe first round ,antibody incubation with S100B Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488). The second round , antibody incubation with GFAP Monoclonal Antibody, Unconjugated (bsm-42001R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red, bs-0295G-BF594). DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
FCM
IHC-P
IHC-F
IF(IHC-P)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-42001r-100ul
Product Name	GFAP Recombinant Antibody
Applications	WB, FCM, IHC-P, IHC-F, IF(IHC-P)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human GFAP protein
Clonality	Recombinant
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2670
Swiss Prot	P14136
Subcellular location	Cytoplasm
Synonyms	Astrocyte Marker; FLJ45472; GFAP; Glial Fibrillary Acidic Protein; Intermediate filament protein; GFAP_HUMAN.
Application Dilution
WB	1:1000-5000
FCM	FCM1:50-100
IHC-P	IHC-P1:200-1000
IHC-F	IHC-F1:200-1000
IF(IHC-P)	IFIHC-P1:200-1000