Help

AIF1 Recombinant Antibody, RBITC Conjugated

Applications

  • WB

Reactivity

  • Mouse
Overview
Catalog # bsm-42057r-rbitc-100uL
Product Name AIF1 Recombinant Antibody, RBITC Conjugated
Applications
WB
Reactivity Mouse
Specifications
Conjugation RBITC
Host Rabbit
Source Recombinant mouse AIF1 protein
Immunogen Range 2-147/147
Clonality Recombinant
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 11629
Swiss Prot O70200
Subcellular location Cytoplasm, Cell membrane
Background Allograft Inflammatory Factor-1 (AIF1)or ionized calcium-binding adaptor molecule 1 (Iba1) is expressed selectively in microglia/macrophages and is a Ca2+-binding peptide produced by activated monocytes and microglial cells. It has been suggested that AIF1 expression is associated with chronic inflammatory processes. AIF1 is expressed by activated monocytes and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts.
Application Dilution
WB 1:500-2000

Frequently asked questions

Which applications is AIF1 Recombinant Antibody, RBITC Conjugated (bsm-42057R-RBITC) validated for, and at what dilution?

bsm-42057R-RBITC is validated for WB(WB) (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does AIF1 Recombinant Antibody, RBITC Conjugated (bsm-42057R-RBITC) react with?

Tested reactivity: Mouse.

How should bsm-42057R-RBITC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise AIF1 Recombinant Antibody, RBITC Conjugated (bsm-42057R-RBITC)?

Recombinant mouse AIF1 protein Immunogen range: 2-147/147.

What are the excitation and emission wavelengths of the RBITC conjugate (bsm-42057R-RBITC)?

RBITC on bsm-42057R-RBITC: excitation / emission 570nm/595nm.