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Myosin VIIa Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with Myosin VIIa monoclonal antibody, unconjugated (bsm-42234R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Myosin VIIa monoclonal antibody, unconjugated (bsm-42234R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Myosin VIIa Monoclonal Antibody, Unconjugated (bsm-42234R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Myosin VIIa Monoclonal Antibody, Unconjugated (bsm-42234R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Myosin VIIa Monoclonal Antibody, Unconjugated (bsm-42234R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Ratbbit IgG antibody (red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Myosin VIIa Monoclonal Antibody, Unconjugated (bsm-42234R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Ratbbit IgG antibody (red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Bioss unconjugated antibody vial

Applications

  • WB

Reactivity

  • Mouse
Overview
Catalog # bsm-42234r
Product Name Myosin VIIa Recombinant Antibody
Applications
WB
Reactivity Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant mouse Myosin VIIa protein
Immunogen Range 861-1035/2215
Clonality Recombinant
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 17921
Swiss Prot P97479
Subcellular location Cytoplasm
Synonyms Deafness autosomal dominant 11; Deafness autosomal recessive 2; DFNA11; DFNB 2; DFNB2; Myo7a; Myosin 7a; Myosin VIIa; MYU7A; NSRD 2; NSRD2; Ush 1B; Ush1b; Usher syndrome 1B.
Background Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. In retina, myosin VIIa may play a role in trafficking of ribbon-synaptic vesicle complexes and renewal of the outer photoreceptors disks. In inner ear, it may maintain the rigidity of stereocilia during the dynamic movements of the bundle.
Application Dilution
WB 1:500-2000

Frequently asked questions

Which applications is Myosin VIIa Recombinant Antibody (bsm-42234R) validated for, and at what dilution?

bsm-42234R is validated for WB(WB) (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Myosin VIIa Recombinant Antibody (bsm-42234R) react with?

Tested reactivity: Mouse.

How should bsm-42234R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise Myosin VIIa Recombinant Antibody (bsm-42234R)?

Recombinant mouse Myosin VIIa protein Immunogen range: 861-1035/2215.

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