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IL7R/CD127 Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IL7R\/CD127 Monoclonal Antibody, Unconjugated (bsm-43043M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IL7R\/CD127 Monoclonal Antibody, Unconjugated (bsm-43043M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IL7R Monoclonal Antibody, Unconjugated(ascites of bsm-43043M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Mouse, SP-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IL7R Monoclonal Antibody, Unconjugated(ascites of bsm-43043M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Mouse, SP-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IL7R Monoclonal Antibody, Unconjugated(ascites of bsm-43043M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Mouse, SP-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IL7R Monoclonal Antibody, Unconjugated(ascites of bsm-43043M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Mouse, SP-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IL7R Monoclonal Antibody, Unconjugated(ascites of bsm-43043M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Mouse, SP-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IL7R Monoclonal Antibody, Unconjugated(ascites of bsm-43043M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Mouse, SP-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IL7R Monoclonal Antibody, Unconjugated(ascites of bsm-43043M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Mouse, SP-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IL7R Monoclonal Antibody, Unconjugated(ascites of bsm-43043M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Mouse, SP-0024) and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • IHC-P
  • IHC-F
  • IF(IHC-P)

Reactivity

  • Human
Overview
Catalog # bsm-43043m
Product Name IL7R/CD127 Monoclonal Antibody
Applications
IHC-P IHC-F IF(IHC-P)
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Recombinant human IL-7R Alpha / CD127 protein
Clonality Monoclonal
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 3575
Swiss Prot P16871
Subcellular location Secreted, Cell membrane
Synonyms IL-7Rα; IL-7Ra; Interleukin-7 receptor subunit alpha; CD 127; CD127; CD127 antigen; CDW127; IL 7R alpha; IL 7R; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL7RA; IL7Ralpha; Interleukin 7 receptor alpha chain; Interleukin 7 receptor; Interleukin 7 receptor isoform H5 6; IL7RA_HUMAN.
Background Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).
Application Dilution
IHC-P 1:50-200
IHC-F IHC-F1:50-200
IF(IHC-P) IFIHC-P1:50-200

Frequently asked questions

Which applications is IL7R/CD127 Monoclonal Antibody (bsm-43043M) validated for, and at what dilution?

bsm-43043M is validated for IHC-P(IHC-P) (1:50-200); IHC-F(IHC-F) (1:50-200); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does IL7R/CD127 Monoclonal Antibody (bsm-43043M) react with?

Tested reactivity: Human.

How should bsm-43043M be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise IL7R/CD127 Monoclonal Antibody (bsm-43043M)?

Recombinant human IL-7R Alpha / CD127 protein

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