PD-L1 (3B11) Monoclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-43073M-BF488 |
| Product Name | PD-L1 (3B11) Monoclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Mouse |
| Source | Recombinant human PD-L1 protein |
| Clonality | Monoclonal |
| Clone # | 3B11 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 29126 |
| Swiss Prot | Q9NZQ7 |
| Subcellular location | Cell membrane |
| Synonyms | Programmed cell death 1 ligand 1; CD274; B7 H; B7 H1; B7 homolog 1; B7-H1; B7H; B7H1; CD 274; CD274 antigen; CD274 molecule; MGC142294; MGC142296; OTTHUMP00000021029; PD L1; PD1L1_HUMAN; PD1L1_Mouse; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; PDL 1; PDL1; Programmed death ligand 1; RGD1566211. |
| Background | This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is PD-L1 (3B11) Monoclonal Antibody, AbBy Fluor-488 Conjugated (bsm-43073M-BF488) validated for, and at what dilution?
bsm-43073M-BF488 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PD-L1 (3B11) Monoclonal Antibody, AbBy Fluor-488 Conjugated (bsm-43073M-BF488) react with?
Tested reactivity: Human.
How should bsm-43073M-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PD-L1 (3B11) Monoclonal Antibody, AbBy Fluor-488 Conjugated (bsm-43073M-BF488)?
Recombinant human PD-L1 protein
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bsm-43073M-BF488)?
AbBy Fluor-488 on bsm-43073M-BF488: excitation / emission 499nm/519nm.