Osteocalcin Monoclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-43106m-cy3-100uL |
| Product Name | Osteocalcin Monoclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Mouse |
| Source | Recombinant human Osteocalcin protein |
| Clonality | Monoclonal |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 632 |
| Swiss Prot | P02818 |
| Synonyms | Osteocalcin Propeptide; Gamma-carboxyglutamic acid-containing protein; Bone Gla-protein; BGLAP; Bone gamma carboxyglutamate protein; BGLAP; BGP; Bone gamma carboxyglutamate gla protein osteocalcin; Bone gamma carboxyglutamate protein; Bone Gla protein; Gamma carboxyglutamic acid containing protein; OC; PMF1; OSTCN_HUMAN. |
| Background | Osteocalcin belongs to the osteocalcin/matrix Gla protein family and constitutes 1 to 2% of the total bone protein. It is a 49 amino acid single chain vitamin K dependent protein, made by osteoblasts, and is a major component of the noncollagenous bone matrix. Post translational modification by a vitamin K dependent carboxylase produces three gamma carboxyglutamic acid residues at positions 17, 21 and 24, giving it a high affinity for calcium. It also binds strongly to apatite. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
Frequently asked questions
Which applications is Osteocalcin Monoclonal Antibody, Cy3 Conjugated (bsm-43106M-Cy3) validated for, and at what dilution?
bsm-43106M-Cy3 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Osteocalcin Monoclonal Antibody, Cy3 Conjugated (bsm-43106M-Cy3) react with?
Tested reactivity: Human.
How should bsm-43106M-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Osteocalcin Monoclonal Antibody, Cy3 Conjugated (bsm-43106M-Cy3)?
Recombinant human Osteocalcin protein
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-43106M-Cy3)?
Cy3 on bsm-43106M-Cy3: excitation / emission 512,550nm/570,615nm.