PGC Monoclonal Antibody, HRP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-43143m-hrp-100uL |
| Product Name | PGC Monoclonal Antibody, HRP Conjugated |
| Applications |
WB
IHC-P
IHC-F
|
| Reactivity | Rat |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | HRP |
| Host | Mouse |
| Source | Recombinant human PGC protein |
| Clonality | Monoclonal |
| Clone # | No |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5225 |
| Swiss Prot | P20142 |
| Synonyms | PEPC_HUMAN; Gastricsin; EC:3.4.23.3; Pepsinogen C; PEPC; PGII; progastricsin; |
| Background | This gene encodes an aspartic proteinase that belongs to the peptidase family A1. The encoded protein is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. This protein is also secreted into the serum. This protein is synthesized as an inactive zymogen that includes a highly basic prosegment. This enzyme is converted into its active mature form at low pH by sequential cleavage of the prosegment that is carried out by the enzyme itself. Polymorphisms in this gene are associated with susceptibility to gastric cancers. Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 1. [provided by RefSeq, Oct 2009]. |
| Application Dilution | |
| WB | 1:500-2000 |
| IHC-P | IHC-P1:100-500 |
| IHC-F | IHC-F1:100-500 |
Frequently asked questions
Which applications is PGC Monoclonal Antibody, HRP Conjugated (bsm-43143M-HRP) validated for, and at what dilution?
bsm-43143M-HRP is validated for WB(WB) (1:500-2000); IHC-P(IHC-P) (1:100-500); IHC-F(IHC-F) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PGC Monoclonal Antibody, HRP Conjugated (bsm-43143M-HRP) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should bsm-43143M-HRP be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PGC Monoclonal Antibody, HRP Conjugated (bsm-43143M-HRP)?
Recombinant human PGC protein