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TFRC Monoclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • FCM
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-43165m-cy3-100uL
Product Name TFRC Monoclonal Antibody, Cy3 Conjugated
Applications
WB FCM IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy3
Host Mouse
Source Recombinant human CD71 protein
Clonality Monoclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 22042
Swiss Prot Q62351
Subcellular location Secreted, Cell membrane
Synonyms ansferrin receptor protein 1; TFRC; CD 71; CD71 antigen; p90; T9; TFR 1; TFR; TFR1; TR; sTfR; Transferrin receptor (p90 CD71); Transferrin Receptor Protein 1; OTTHUMP00000208523; OTTHUMP00000208524; OTTHUMP00000208525; TRFR; TFR1_HUMAN.
Background This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
Application Dilution
WB 1:500-2000
FCM FCM2ug/Test
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is TFRC Monoclonal Antibody, Cy3 Conjugated (bsm-43165M-Cy3) validated for, and at what dilution?

bsm-43165M-Cy3 is validated for WB(WB) (1:500-2000); FCM(FCM) (2ug/Test); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TFRC Monoclonal Antibody, Cy3 Conjugated (bsm-43165M-Cy3) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-43165M-Cy3 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TFRC Monoclonal Antibody, Cy3 Conjugated (bsm-43165M-Cy3)?

Recombinant human CD71 protein

What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-43165M-Cy3)?

Cy3 on bsm-43165M-Cy3: excitation / emission 512,550nm/570,615nm.