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CD16A Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD16A Monoclonal Antibody, Unconjugated (bsm-43212M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD16A Monoclonal Antibody, Unconjugated (bsm-43212M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD16A Monoclonal Antibody, Unconjugated (bsm-43212M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD16A Monoclonal Antibody, Unconjugated (bsm-43212M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD16A Monoclonal Antibody, Unconjugated (bsm-43212M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD16A Monoclonal Antibody, Unconjugated (bsm-43212M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • IHC-P
  • IHC-F
  • IF(IHC-P)

Reactivity

  • Human
Overview
Catalog # bsm-43212m
Product Name CD16A Monoclonal Antibody
Applications
IHC-P IHC-F IF(IHC-P)
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Recombinant human Fc gamma RIIIA/CD16a (F176) Protein
Clonality Monoclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 2214
Swiss Prot P08637
Subcellular location Secreted, Cell membrane
Synonyms FCG3A_HUMAN; Low affinity immunoglobulin gamma Fc region receptor III-A; CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc receptor III-2; CD_antigen: D16a; FCGR3A; CD16A; FCG3; FCGR3; IGFR3.
Application Dilution
IHC-P 1:100-500
IHC-F IHC-F1:100-500
IF(IHC-P) IFIHC-P1:100-500

Frequently asked questions

Which applications is CD16A Monoclonal Antibody (bsm-43212M) validated for, and at what dilution?

bsm-43212M is validated for IHC-P(IHC-P) (1:100-500); IHC-F(IHC-F) (1:100-500); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CD16A Monoclonal Antibody (bsm-43212M) react with?

Tested reactivity: Human.

How should bsm-43212M be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CD16A Monoclonal Antibody (bsm-43212M)?

Recombinant human Fc gamma RIIIA/CD16a (F176) Protein

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