E-Selectin Monoclonal Antibody

$100 ng rhE-Selectin-His protein (bs-43646P) per lane probed with E-Selectin monoclonal antibody respectively, unconjugated (bsm-43646M) at 1:200 dilution and 4\u00b0C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.$

100 ng rhE-Selectin-His protein (bs-43646P) per lane probed with E-Selectin monoclonal antibody respectively, unconjugated (bsm-43646M) at 1:200 dilution and 4\u00b0C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with E-Selectin Monoclonal Antibody, Unconjugated(bsm-43646M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with E-Selectin Monoclonal Antibody, Unconjugated(bsm-43646M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with E-Selectin Monoclonal Antibody, Unconjugated(bsm-43646M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with E-Selectin Monoclonal Antibody, Unconjugated(bsm-43646M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human

Overview
Catalog #	bsm-43646m
Product Name	E-Selectin Monoclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human E-Selectin protein
Clonality	Monoclonal
Clone #	1G1
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6401
Swiss Prot	P16581
Subcellular location	Cell membrane
Synonyms	CD 62E; CD62E; CD62E antigen; CD62 antigen-like family member E; E selectin; ELAM 1; ELAM; ELAM-1; ELAM1; Endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; LECAM 2; LECAM2; Leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; LYAM2_HUMAN; SELE; Selectin E; SelectinE.
Background	Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF