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CD33 Monoclonal Antibody

The PBMC (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Mouse Anti-CD33 antibody (bsm-43662M,1:100); Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The PBMC (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Mouse Anti-CD33 antibody (bsm-43662M,1:100); Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The Jurkat (Negative Control) (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.) , followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Mouse Anti-CD33 antibody (bsm-43662M,1:100); Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The Jurkat (Negative Control) (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.) , followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Mouse Anti-CD33 antibody (bsm-43662M,1:100); Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • Flow-Cyt

Reactivity

  • Human
Overview
Catalog # bsm-43662m-100ul
Product Name CD33 Monoclonal Antibody
Applications
FLOW-CYT
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Recombinant human CD33 protein
Clonality Monoclonal
Clone # 2E3
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 945
Swiss Prot P20138
Subcellular location Cell membrane
Synonyms CD33rSiglec; SIGLEC-3; SIGLEC3; p67; gp67; CD33_HUMAN; CD33; Sialic acid-binding Ig-like lectin 3 (Siglec-3); CD33_MOUSE;
Application Dilution
Flow-Cyt =1ug/Test

Frequently asked questions

Which applications is CD33 Monoclonal Antibody (bsm-43662M) validated for, and at what dilution?

bsm-43662M is validated for Flow-Cyt (Flow-Cyt=1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CD33 Monoclonal Antibody (bsm-43662M) react with?

Tested reactivity: Human.

How should bsm-43662M be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CD33 Monoclonal Antibody (bsm-43662M)?

Recombinant human CD33 protein

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