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CD30 Monoclonal Antibody

25 ug total protein per lane of various lysates (see on figure) probed with CD30 monoclonal antibody, unconjugated (bsm-43683M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with CD30 monoclonal antibody, unconjugated (bsm-43683M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD30 Monoclonal Antibody, Unconjugated (bsm-43683M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD30 Monoclonal Antibody, Unconjugated (bsm-43683M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Diffuse large B-cell lymphoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD30 Monoclonal Antibody, Unconjugated (bsm-43683M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Diffuse large B-cell lymphoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD30 Monoclonal Antibody, Unconjugated (bsm-43683M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD30 Monoclonal Antibody, Unconjugated (bsm-43683M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD30 Monoclonal Antibody, Unconjugated (bsm-43683M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB

Reactivity

  • Human
Overview
Catalog # bsm-43683m-100ul
Product Name CD30 Monoclonal Antibody
Applications
WB
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Recombinant human CD30 protein
Clonality Monoclonal
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 943
Swiss Prot P28908
Subcellular location Cytoplasm, Cell membrane
Synonyms CD 30; CD30 antigen; CD30L receptor; Cytokine receptor CD30; D1S166E; KI 1; Ki 1 antigen; Ki-1 antigen; KI1; Lymphocyte activation antigen CD30; TNFRSF 8; TNFRSF8; TNR8_HUMAN; Tumor Necrosis Factor Receptor Superfamily Member 8; Tumor Necrosis Factor Receptor Superfamily Member 8.
Application Dilution
WB =1:500-2000

Frequently asked questions

Which applications is CD30 Monoclonal Antibody (bsm-43683M) validated for, and at what dilution?

bsm-43683M is validated for WB (WB=1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CD30 Monoclonal Antibody (bsm-43683M) react with?

Tested reactivity: Human.

How should bsm-43683M be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CD30 Monoclonal Antibody (bsm-43683M)?

Recombinant human CD30 protein

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