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CD14 Monoclonal Antibody

1% SDS hot lysates, 25 ug total protein per lane of various lysates (see on figure) probed with CD14 monoclonal antibody, unconjugated (bsm-43686M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
1% SDS hot lysates, 25 ug total protein per lane of various lysates (see on figure) probed with CD14 monoclonal antibody, unconjugated (bsm-43686M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (bsm-43686M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (bsm-43686M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (bsm-43686M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (bsm-43686M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (bsm-43686M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (bsm-43686M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (bsm-43686M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (bsm-43686M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (bsm-43686M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (bsm-43686M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (bsm-43686M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (bsm-43686M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (bsm-43686M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (bsm-43686M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (bsm-43686M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (bsm-43686M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (bsm-43686M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD14 Monoclonal Antibody, Unconjugated (bsm-43686M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB

Reactivity

  • Human
Overview
Catalog # bsm-43686m-100ul
Product Name CD14 Monoclonal Antibody
Applications
WB
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Recombinant human CD14 protein
Clonality Monoclonal
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 929
Swiss Prot P08571
Subcellular location Cell membrane
Synonyms CD14 antigen; CD14 molecule; Lipopolysaccharide receptor; LPSR; Monocyte Differentiation Antigen 14; Monocyte differentiation antigen CD14; Myeloid cell specific leucine rich glycoprotein; CD14_MOUSE; Myeloid cell-specific leucine-rich glycoprotein.
Application Dilution
WB =1:1000-5000

Frequently asked questions

Which applications is CD14 Monoclonal Antibody (bsm-43686M) validated for, and at what dilution?

bsm-43686M is validated for WB (WB=1:1000-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CD14 Monoclonal Antibody (bsm-43686M) react with?

Tested reactivity: Human.

How should bsm-43686M be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CD14 Monoclonal Antibody (bsm-43686M)?

Recombinant human CD14 protein

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