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VEGFA (5F4) Monoclonal Antibody

Applications

  • FCM
  • IHC-P
  • IHC-F
  • IF(ICC)
  • IF

Reactivity

  • Human
Overview
Catalog # bsm-4572M
Product Name VEGFA (5F4) Monoclonal Antibody
Applications
FCM IHC-P IHC-F IF(ICC) IF
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source KLH conjugated synthetic peptide derived from human VEGF-A
Clonality Monoclonal
Clone # 5F4
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 7422
Swiss Prot P15692
Synonyms Vascuoar endothelial growth factor A; vascular endothelial growth factor A isoform 2 precursor; Vegf; VEGF164; VEGFA; MGC70609; MVCD1; VEGF; VPF.
Background This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms. [provided by RefSeq].
Application Dilution
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(ICC) 1:50-200
IF

Frequently asked questions

Which applications is VEGFA (5F4) Monoclonal Antibody (bsm-4572M) validated for, and at what dilution?

bsm-4572M is validated for FCM (1:20-100); IHC-P (1:200-400); IHC-F (1:100-500); IF(ICC) (1:50-200); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does VEGFA (5F4) Monoclonal Antibody (bsm-4572M) react with?

Tested reactivity: Human.

How should bsm-4572M be stored?

Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise VEGFA (5F4) Monoclonal Antibody (bsm-4572M)?

KLH conjugated synthetic peptide derived from human VEGF-A