VEGFA (5F4) Monoclonal Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-4572M-FITC |
| Product Name | VEGFA (5F4) Monoclonal Antibody, FITC Conjugated |
| Applications |
FCM
IF(ICC)
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | FITC |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human VEGF-A |
| Clonality | Monoclonal |
| Clone # | 5F4 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7422 |
| Swiss Prot | P15692 |
| Synonyms | Vascuoar endothelial growth factor A; vascular endothelial growth factor A isoform 2 precursor; Vegf; VEGF164; VEGFA; MGC70609; MVCD1; VEGF; VPF. |
| Background | This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms. [provided by RefSeq]. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(ICC) | 1:50-200 |
| IF | |
Frequently asked questions
Which applications is VEGFA (5F4) Monoclonal Antibody, FITC Conjugated (bsm-4572M-FITC) validated for, and at what dilution?
bsm-4572M-FITC is validated for FCM (1:20-100); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does VEGFA (5F4) Monoclonal Antibody, FITC Conjugated (bsm-4572M-FITC) react with?
Tested reactivity: Human.
How should bsm-4572M-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise VEGFA (5F4) Monoclonal Antibody, FITC Conjugated (bsm-4572M-FITC)?
KLH conjugated synthetic peptide derived from human VEGF-A
What are the excitation and emission wavelengths of the FITC conjugate (bsm-4572M-FITC)?
FITC on bsm-4572M-FITC: excitation / emission 494nm/518nm.