CEA (5F2) Monoclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-4793M-Cy5-100uL |
| Product Name | CEA (5F2) Monoclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from Human CEA/CEACAM5 |
| Immunogen Range | 531-630/702 |
| Clonality | Monoclonal |
| Clone # | 5F2 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1048 |
| Synonyms | Carcino Embryonic Antigen CEA; CEACAM 5; CEACAM-5; Carcinoembryonic antigen; Carcinoembryonic antigen related cell adhesion molecule 5; CD66e; CD66e antigen; CEA; CEACAM5; DKFZp781M2392; Meconium antigen 100. |
| Background | CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glyco-protein (PSG 1-PSG 11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/ CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF | 1:50-200 |
Frequently asked questions
Which applications is CEA (5F2) Monoclonal Antibody, Cy5 Conjugated (bsm-4793M-Cy5) validated for, and at what dilution?
bsm-4793M-Cy5 is validated for WB (1:500-2000); IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CEA (5F2) Monoclonal Antibody, Cy5 Conjugated (bsm-4793M-Cy5) react with?
Tested reactivity: Human.
How should bsm-4793M-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CEA (5F2) Monoclonal Antibody, Cy5 Conjugated (bsm-4793M-Cy5)?
KLH conjugated synthetic peptide derived from Human CEA/CEACAM5 Immunogen range: 531-630/702.
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-4793M-Cy5)?
Cy5 on bsm-4793M-Cy5: excitation / emission 625,650nm/670nm.