TRIM21 Monoclonal Antibody

$Hela (H) cells were treated with or without hIFN-\u03b11 (10 ng\/ml) for 16 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with TRIM21 monoclonal antibody, unconjugated (bsm-49248M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

Hela (H) cells were treated with or without hIFN-\u03b11 (10 ng\/ml) for 16 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with TRIM21 monoclonal antibody, unconjugated (bsm-49248M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (TRIM21) monoclonal Antibody, unconjugated (bsm-49248M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (TRIM21) monoclonal Antibody, unconjugated (bsm-49248M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Applications

WB
ICC/IF

Reactivity

Human

Overview
Catalog #	bsm-49248m
Product Name	TRIM21 Monoclonal Antibody
Applications	WB, ICC/IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human TRIM21 protein
Clonality	Monoclonal
Clone #	12G3
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6737
Swiss Prot	P19474
Subcellular location	Cytoplasm, Nucleus
Synonyms	52 kDa ribonucleoprotein autoantigen Ro/SS-A; 52 kDa Ro protein; 52kD Ro/SSA autoantigen; Autoantigen Ro/SSA, 52-KD; E3 ubiquitin-protein ligase TRIM21; RING finger protein 81; RNF81; Ro 52; Ro(SS-A); Ro52; RO52_HUMAN; Sicca syndrome antigen A; Sjoegren syndrome type A antigen; Sjogren syndrome antigen A1; Sjogren syndrome type A antigen; SS-A; SSA; SSA1: Sjogren syndrome antigen A1(52kDa ribonucleoprotein autoantigen SS-A/Ro); TRIM21; Tripartite motif protein TRIM21; Tripartite motif-containing 21; Tripartite motif-containing protein 21.
Application Dilution
WB	1:300-5000
ICC/IF	1:50-100