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beta actin (8H10D10) Monoclonal Antibody, PE Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-51011m-pe-100ul
Product Name beta actin (8H10D10) Monoclonal Antibody, PE Conjugated
Applications
WB IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation PE
Host Mouse
Source ACTB recombinant protein is used to produce this monoclonal antibody.
Clonality Monoclonal
Clone # 8H10D10
Isotype IgG
Concentration 0.5ug/ul
Purification Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purifie
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 60
Swiss Prot P60709
Synonyms BRWS1; PS1TP5BP1; Actin, cytoplasmic 1; Beta-actin; ACTB
Background Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Application Dilution
WB 1:1000-5000
IF 1:200-1000

Frequently asked questions

Which applications is beta actin (8H10D10) Monoclonal Antibody, PE Conjugated (bsm-51011M-PE) validated for, and at what dilution?

bsm-51011M-PE is validated for WB (1:1000-5000); IF (1:200-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does beta actin (8H10D10) Monoclonal Antibody, PE Conjugated (bsm-51011M-PE) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-51011M-PE be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise beta actin (8H10D10) Monoclonal Antibody, PE Conjugated (bsm-51011M-PE)?

ACTB recombinant protein is used to produce this monoclonal antibody.

What are the excitation and emission wavelengths of the PE conjugate (bsm-51011M-PE)?

PE on bsm-51011M-PE: excitation / emission 496,564nm/578nm.