UBC9 (7A9) Monoclonal Antibody

$Lane 1: human heart lysate; Lane 2: A431 whole cell lysate; Lane 3: Hela whole cell lysate; Lane 4: Jurkat whole cell lysate; Lane 5: NIH\/3T3 whole cell lysate; Lane 6: PC-12 whole cell lysate at 20 \u00b5g per lane; probed with bsm-51021M UBC9 (67AT1273.95.90) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.$

Lane 1: human heart lysate; Lane 2: A431 whole cell lysate; Lane 3: Hela whole cell lysate; Lane 4: Jurkat whole cell lysate; Lane 5: NIH\/3T3 whole cell lysate; Lane 6: PC-12 whole cell lysate at 20 \u00b5g per lane; probed with bsm-51021M UBC9 (67AT1273.95.90) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-51021M
Product Name	UBC9 (7A9) Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This UBC9 antibody was raised using purified GST-UBC9 fusion protein.
Clonality	Monoclonal
Clone #	7A9
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	7329
Swiss Prot	P63279
Synonyms	P18; UBC9; C358B7.1; SUMO-conjugating enzyme UBC9; SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I; UBE2I; UBCE9
Background	Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'.
Application Dilution
WB	1:300-5000