ITA6 (9C5) Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded human skin carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ITA6 (129CT49.6.5) Monoclonal Antibody (bsm-51027M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human skin carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ITA6 (129CT49.6.5) Monoclonal Antibody (bsm-51027M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Applications

WB
FCM
IHC-P
IF

Reactivity

Human

Overview
Catalog #	bsm-51027M
Product Name	ITA6 (9C5) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	ITA6 recombinant protein is used to produce this monoclonal antibody.
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	3655
Swiss Prot	P23229
Synonyms	CD49f; VLA-6; ITGA6B; Integrin alpha-6; CD49 antigen-like family member F; ITGA6
Background	Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760).
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF