NTRK1 (5C1) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51044M-100uL |
| Product Name | NTRK1 (5C1) Monoclonal Antibody |
| Applications |
WB
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | This NTRK1 Monoclonal antibody was raised using purified His-tagged recombinant human NTRK1. |
| Clonality | Monoclonal |
| Clone # | 5C1 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 4914 |
| Swiss Prot | P04629 |
| Synonyms | MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA; High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; TRK1-transforming tyrosine kinase protein; Tropomyosin-related kinase A; Tyrosine kinase receptor; Tyrosine kinase receptor A; gp140trk; NTRK1 |
| Background | Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand, it can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors. Isoform TrkA-III is resistant to NGF, constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade. Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is NTRK1 (5C1) Monoclonal Antibody (bsm-51044M) validated for, and at what dilution?
bsm-51044M is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NTRK1 (5C1) Monoclonal Antibody (bsm-51044M) react with?
Tested reactivity: Human, Mouse.
How should bsm-51044M be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NTRK1 (5C1) Monoclonal Antibody (bsm-51044M)?
This NTRK1 Monoclonal antibody was raised using purified His-tagged recombinant human NTRK1.