ATG4D (2C1) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51061M-100uL |
| Product Name | ATG4D (2C1) Monoclonal Antibody |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | This ATG4D monoclonal antibody is generated from mouse immunized with ATG4D recombinant protein. |
| Clonality | Monoclonal |
| Clone # | 2C1 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 84971 |
| Swiss Prot | Q86TL0 |
| Synonyms | APG4D; AUTL4; APG4-D; Cysteine protease ATG4D; AUT-like 4 cysteine endopeptidase; Autophagin-4; Autophagy-related cysteine endopeptidase 4; Autophagy-related protein 4 homolog D; ATG4D |
| Background | Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms. Cysteine protease ATG4D, mitochondrial: Plays a role as an autophagy regulator that links mitochondrial dysfunction with apoptosis. The mitochondrial import of ATG4D during cellular stress and differentiation may play important roles in the regulation of mitochondrial physiology, ROS, mitophagy and cell viability. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is ATG4D (2C1) Monoclonal Antibody (bsm-51061M) validated for, and at what dilution?
bsm-51061M is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ATG4D (2C1) Monoclonal Antibody (bsm-51061M) react with?
Tested reactivity: Human.
How should bsm-51061M be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ATG4D (2C1) Monoclonal Antibody (bsm-51061M)?
This ATG4D monoclonal antibody is generated from mouse immunized with ATG4D recombinant protein.