ELAVL1 (4C3) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51064M |
| Product Name | ELAVL1 (4C3) Monoclonal Antibody |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | This ELAVL1 monoclonal antibody is generated from mouse immunized with ELAVL1 recombinant protein. |
| Clonality | Monoclonal |
| Clone # | 4C3 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purifie |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 1994 |
| Swiss Prot | Q15717 |
| Synonyms | HUR; Hua; MelG; ELAV1; ELAV-like protein 1; Hu-antigen R; ELAVL1 |
| Background | RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (PubMed:14517288, PubMed:18285462). Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation (By similarity). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed:8626503, PubMed:17632515, PubMed:18285462, PubMed:23519412). Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro (PubMed:8626503). With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is ELAVL1 (4C3) Monoclonal Antibody (bsm-51064M) validated for, and at what dilution?
bsm-51064M is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ELAVL1 (4C3) Monoclonal Antibody (bsm-51064M) react with?
Tested reactivity: Human.
How should bsm-51064M be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ELAVL1 (4C3) Monoclonal Antibody (bsm-51064M)?
This ELAVL1 monoclonal antibody is generated from mouse immunized with ELAVL1 recombinant protein.