MAP4K3 (9T1) Monoclonal Antibody

$Lane 1: HepG2 Cell lysates; Lane 2: MCF-7 Cell lysates; probed with MAP4K3 (219CT8.3.1) Monoclonal Antibody, unconjugated (bsm-51067M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: HepG2 Cell lysates; Lane 2: MCF-7 Cell lysates; probed with MAP4K3 (219CT8.3.1) Monoclonal Antibody, unconjugated (bsm-51067M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed, paraffin embedded Human skeletal muscle tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with MAP4K3 (219CT8.3.1) Monoclonal Antibody (bsm-51067M) at 1:100 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human skeletal muscle tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with MAP4K3 (219CT8.3.1) Monoclonal Antibody (bsm-51067M) at 1:100 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Applications

WB
IHC-P

Reactivity

Human

Overview
Catalog #	bsm-51067M
Product Name	MAP4K3 (9T1) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This MAP4K3 monoclonal antibody is generated from mouse immunized with MAP4K3 recombinant protein.
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgM
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	8491
Swiss Prot	Q8IVH8
Synonyms	GLK; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1; Mitogen-activated protein kinase kinase kinase kinase 3; Germinal center kinase-related protein kinase; MAPK/ERK kinase kinase kinase 3; MEK kinase kinase 3; MAP4K3
Background	May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400