RAB8A (8A4) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51077M-100uL |
| Product Name | RAB8A (8A4) Monoclonal Antibody |
| Applications |
WB
ELISA
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | This RAB8A monoclonal antibody is generated from mouse immunized with RAB8A recombinant protein. |
| Clonality | Monoclonal |
| Clone # | 8A4 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purifie |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 4218 |
| Swiss Prot | P61006 |
| Synonyms | MEL; RAB8; Ras-related protein Rab-8A; Oncogene c-mel; RAB8A |
| Background | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Plays an important role in ciliogenesis. Together with MICALL2, may also regulate adherens junction assembly. May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:50-200 |
Frequently asked questions
Which applications is RAB8A (8A4) Monoclonal Antibody (bsm-51077M) validated for, and at what dilution?
bsm-51077M is validated for WB (1:300-5000); ELISA (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RAB8A (8A4) Monoclonal Antibody (bsm-51077M) react with?
Tested reactivity: Human, Mouse.
How should bsm-51077M be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RAB8A (8A4) Monoclonal Antibody (bsm-51077M)?
This RAB8A monoclonal antibody is generated from mouse immunized with RAB8A recombinant protein.