SOX2 (7C4) Monoclonal Antibody

$NCCIT cell lysate probed with bsm-51142M SOX2 (7C4) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.$

NCCIT cell lysate probed with bsm-51142M SOX2 (7C4) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

$Lane 1: SOX2 Protein Isolate; probed with SOX2 (7C4) Monoclonal Antibody, unconjugated (bsm-51142M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: SOX2 Protein Isolate; probed with SOX2 (7C4) Monoclonal Antibody, unconjugated (bsm-51142M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with SOX2 (7C4) Monoclonal Antibody (bsm-51142M) at 1:50 (37\u2103 for 1hr) in A549 cells (green). Cells were counterstained with Alexa Fluor\u00ae 555 (red) conjugated Phalloidin. Cells were fixed in 4% paraformaldehyde for 20min and permeabilized with 0.1% Triton X100\/PBS for 10min.$

IF(ICC) staining with SOX2 (7C4) Monoclonal Antibody (bsm-51142M) at 1:50 (37\u2103 for 1hr) in A549 cells (green). Cells were counterstained with Alexa Fluor\u00ae 555 (red) conjugated Phalloidin. Cells were fixed in 4% paraformaldehyde for 20min and permeabilized with 0.1% Triton X100\/PBS for 10min.

$Paraformaldehyde-fixed and paraffin-embedded Human Lung Cancer tissue incubated with SOX2 (7C4) Monoclonal Antibody (bsm-51142M) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Lung Cancer tissue incubated with SOX2 (7C4) Monoclonal Antibody (bsm-51142M) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Lane 1: 293 Cells nontransfected; Lane 2: 293 Cells transiently transfected with SOX2 gene; Lane 3: Mouse Testis; Probed with SOX2 (7C4) Monoclonal Antibody (bsm-51142M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: 293 Cells nontransfected; Lane 2: 293 Cells transiently transfected with SOX2 gene; Lane 3: Mouse Testis; Probed with SOX2 (7C4) Monoclonal Antibody (bsm-51142M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Flow cytometric analysis of NCI-H460 cells with SOX2 (7C4) Monoclonal Antibody (bsm-51142M) at a 1:50 dilution (red) compared with a negative control (cells without incubation with primary antibody; blue).

Applications

WB
FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-51142M
Product Name	SOX2 (7C4) Monoclonal Antibody
Applications	WB FCM IHC-P IF(IHC-P) IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	SOX2 recombinant protein
Clonality	Monoclonal
Clone #	7C4
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	6657
Swiss Prot	P48431
Subcellular location	Nucleus
Synonyms	Transcription factor SOX-2; SOX2
Background	Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200