CTNNB1 (6C3) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51160M-100uL |
| Product Name | CTNNB1 (6C3) Monoclonal Antibody |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | This CTNNB1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 646-673 amino acids from human CTNNB1. |
| Clonality | Monoclonal |
| Clone # | 6C3 |
| Isotype | IgM |
| Concentration | Lot Dependent |
| Purification | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 1499 |
| Swiss Prot | P35222 |
| Synonyms | CTNNB; MRD19; armadillo; Catenin beta-1; Beta-catenin; CTNNB1; OK/SW-cl.35; PRO2286 |
| Background | Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22647378, PubMed:22699938, PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is CTNNB1 (6C3) Monoclonal Antibody (bsm-51160M) validated for, and at what dilution?
bsm-51160M is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CTNNB1 (6C3) Monoclonal Antibody (bsm-51160M) react with?
Tested reactivity: Human.
How should bsm-51160M be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CTNNB1 (6C3) Monoclonal Antibody (bsm-51160M)?
This CTNNB1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 646-673 amino acids from human CTNNB1.