IL1B (4C1) Monoclonal Antibody

$RAW 264.7 (M) cells were treated with or without LPS (100ng\/ml) for 6 h, A added (300ng\/ml) for 3 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with IL1B | IL-1 beta monoclonal antibody, unconjugated (bsm-51186M) at 1:300 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

RAW 264.7 (M) cells were treated with or without LPS (100ng\/ml) for 6 h, A added (300ng\/ml) for 3 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with IL1B | IL-1 beta monoclonal antibody, unconjugated (bsm-51186M) at 1:300 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$THP-1 (H) cells were treated with or without TPA (80nM) for 24 h, LPS (100ng\/ml) for 6 h, Brefeldin A (300ng\/ml) for 3 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with IL1B | IL-1 beta monoclonal antibody, unconjugated (bsm-51186M) at 1:300 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

THP-1 (H) cells were treated with or without TPA (80nM) for 24 h, LPS (100ng\/ml) for 6 h, Brefeldin A (300ng\/ml) for 3 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with IL1B | IL-1 beta monoclonal antibody, unconjugated (bsm-51186M) at 1:300 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bsm-51186M
Product Name	IL1B (4C1) Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human IL-1 beta protein
Clonality	Monoclonal
Clone #	6G7
Isotype	IgG1, k
Concentration	1mg/ml
Purification	purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3553
Swiss Prot	P01584
Subcellular location	Secreted
Synonyms	IL-1; IL1-BETA; IL1F2; IL1beta; IL-1beta; Il-1b; IL-1F2; IL1B_HUMAN; IL1B; IL-1 beta; Catabolin; IL1B_MOUSE; IL1B_RAT; interleukin 1 beta
Background	Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
Application Dilution
WB	1:300-5000