H2AX (8C5) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51222M-100uL |
| Product Name | H2AX (8C5) Monoclonal Antibody |
| Applications |
WB
IHC-F
IF
IHC-P
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | This H2AFX antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 115-143 amino acids from the C-terminal region of human H2AFX. |
| Immunogen Range | 100-143/143 |
| Clonality | Monoclonal |
| Clone # | 8C5 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purifie |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 3014 |
| Swiss Prot | P16104 |
| Synonyms | H2AX; H2A.X; H2A/X; Histone H2AX; Histone H2A.X; H2AFX |
| Background | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-F | 1:100-500 |
| IF | 1:50-200 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is H2AX (8C5) Monoclonal Antibody (bsm-51222M) validated for, and at what dilution?
bsm-51222M is validated for WB (1:300-5000); IHC-F (1:100-500); IF (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does H2AX (8C5) Monoclonal Antibody (bsm-51222M) react with?
Tested reactivity: Human.
How should bsm-51222M be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise H2AX (8C5) Monoclonal Antibody (bsm-51222M)?
This H2AFX antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 115-143 amino acids from the C-terminal region of human H2AFX. Immunogen range: 100-143/143.