PINK1 (8T5) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51265m-100uL |
| Product Name | PINK1 (8T5) Monoclonal Antibody |
| Applications |
WB
IHC-F
IF
IHC-P
ICC/IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Recombinant PINK1 protein was used to produced this monoclonal antibody. |
| Clonality | Monoclonal |
| Clone # | 8T5 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 65018 |
| Swiss Prot | Q9BXM7 |
| Synonyms | BRPK; PARK6; Serine/threonine-protein kinase PINK1, mitochondrial; PTEN-induced putative kinase protein 1; PINK1 |
| Background | Protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PARK2. Targets PARK2 to dysfunctional depolarized mitochondria through the phosphorylation of MFN2. Activates PARK2 in 2 steps: (1) by mediating phosphorylation at 'Ser-65' of PARK2 and (2) mediating phosphorylation of ubiquitin, converting PARK2 to its fully-active form (PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:25527291). |
| Application Dilution | |
| WB | 1:200-1000 |
| IHC-F | 1:100-500 |
| IF | 1:20-200 |
| IHC-P | 1:100-500 |
| ICC/IF | 1:50-100 |
Frequently asked questions
Which applications is PINK1 (8T5) Monoclonal Antibody (bsm-51265M) validated for, and at what dilution?
bsm-51265M is validated for WB (1:200-1000); IHC-F (1:100-500); IF (1:20-200); IHC-P (1:100-500); ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PINK1 (8T5) Monoclonal Antibody (bsm-51265M) react with?
Tested reactivity: Human.
How should bsm-51265M be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PINK1 (8T5) Monoclonal Antibody (bsm-51265M)?
Recombinant PINK1 protein was used to produced this monoclonal antibody.