CHK1 (2G1D5) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51266M-100uL |
| Product Name | CHK1 (2G1D5) Monoclonal Antibody |
| Applications |
WB
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | This monoclonal antibody is generated from mice immunized with Ni-NTA purified recombinant protein CHK1 expressed in E. Coli strain M15. |
| Clonality | Monoclonal |
| Clone # | 2G1D5 |
| Isotype | IgM |
| Concentration | Lot Dependent |
| Purification | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purifie |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 1111 |
| Swiss Prot | O14757 |
| Synonyms | CHK1; Serine/threonine-protein kinase Chk1; CHK1 checkpoint homolog; Cell cycle checkpoint kinase; Checkpoint kinase-1; CHEK1 |
| Background | Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Also phosphorylates NEK6. Binds to and phosphorylates RAD51 at 'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, thereby promoting DNA repair by homologous recombination. Phosphorylates multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation. Also promotes repair of DNA cross-links through phosphorylation of FANCE. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may enhance chromatin assembly both in the presence or absence of DNA damage. May also play a role in replication fork maintenance through regulation of PCNA. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is CHK1 (2G1D5) Monoclonal Antibody (bsm-51266M) validated for, and at what dilution?
bsm-51266M is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CHK1 (2G1D5) Monoclonal Antibody (bsm-51266M) react with?
Tested reactivity: Human, Mouse.
How should bsm-51266M be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CHK1 (2G1D5) Monoclonal Antibody (bsm-51266M)?
This monoclonal antibody is generated from mice immunized with Ni-NTA purified recombinant protein CHK1 expressed in E. Coli strain M15.