MAFK (8C5) Monoclonal Antibody

$Lane 1: MAFK Cell lysates; probed with MAFK (8C5) Monoclonal Antibody, unconjugated (bsm-51274M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: MAFK Cell lysates; probed with MAFK (8C5) Monoclonal Antibody, unconjugated (bsm-51274M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Hela cells probed with using MAFK (8C5) Monoclonal Antibody (green) unconjugated bsm-51274M at 1:25 for 30 minutes followed by A488 secondary antibody at 1:400 compared to an isotype control of mouse IgG1(blue).

Applications

Reactivity

Human

Overview
Catalog #	bsm-51274M
Product Name	MAFK (8C5) Monoclonal Antibody
Applications	WB, FCM
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This MAFK antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human MAFK.
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	7975
Swiss Prot	O60675
Synonyms	P18; NFE2U; Transcription factor MafK; Erythroid transcription factor NF-E2 p18 subunit; MAFK
Background	Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.
Application Dilution
WB	1:300-5000
FCM	1:20-100