JUN (6C7) Monoclonal Antibody

$Lane 1: 293 Cell lysates; Lane 2: NIH\/3T3 Cell lysates; probed with JUN (6C7) Monoclonal Antibody, unconjugated (bsm-51278M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: 293 Cell lysates; Lane 2: NIH\/3T3 Cell lysates; probed with JUN (6C7) Monoclonal Antibody, unconjugated (bsm-51278M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bsm-51278M
Product Name	JUN (6C7) Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from human.
Clonality	Monoclonal
Clone #	6C7
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	3725
Swiss Prot	P05412
Synonyms	AP1; AP-1; c-Jun; Transcription factor AP-1; Activator protein 1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39; JUN
Background	Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
Application Dilution
WB	1:300-5000