RAB13 (5C9) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51357M-100uL |
| Product Name | RAB13 (5C9) Monoclonal Antibody |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | This RAB13 antibody is generated from a mouse immunized with a recombinant protein between 1-203 amino acids from human RAB13. |
| Clonality | Monoclonal |
| Clone # | 5C9 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 5872 |
| Swiss Prot | P51153 |
| Synonyms | GIG4; Ras-related protein Rab-13; Cell growth-inhibiting gene 4 protein; RAB13 |
| Background | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in endocytic recycling and regulates the transport to the plasma membrane of transmembrane proteins like the tight junction protein OCLN/occludin. Thereby, it regulates the assembly and the activity of tight junctions. Moreover, it may also regulate tight junction assembly by activating the PKA signaling pathway and by reorganizing the actin cytoskeleton through the activation of the downstream effectors PRKACA and MICALL2 respectively. Through its role in tight junction assembly, may play a role in the establishment of Sertoli cell barrier. Plays also a role in angiogenesis through regulation of endothelial cells chemotaxis. Also involved in neurite outgrowth. Has also been proposed to play a role in post-Golgi membrane trafficking from the TGN to the recycling endosome. Finally, it has been involved in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore may play a role in glucose homeostasis. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is RAB13 (5C9) Monoclonal Antibody (bsm-51357M) validated for, and at what dilution?
bsm-51357M is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RAB13 (5C9) Monoclonal Antibody (bsm-51357M) react with?
Tested reactivity: Human.
How should bsm-51357M be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RAB13 (5C9) Monoclonal Antibody (bsm-51357M)?
This RAB13 antibody is generated from a mouse immunized with a recombinant protein between 1-203 amino acids from human RAB13.