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PGK1 Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PGK1 Monoclonal Antibody, Unconjugated (bsm-51374M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PGK1 Monoclonal Antibody, Unconjugated (bsm-51374M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PGK1 Monoclonal Antibody, Unconjugated (bsm-51374M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PGK1 Monoclonal Antibody, Unconjugated (bsm-51374M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with PGK1 monoclonal antibody, unconjugated (bsm-51374M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with PGK1 monoclonal antibody, unconjugated (bsm-51374M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PGK1 Monoclonal Antibody, Unconjugated (bsm-51374M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PGK1 Monoclonal Antibody, Unconjugated (bsm-51374M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PGK1 Monoclonal Antibody, Unconjugated (bsm-51374M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PGK1 Monoclonal Antibody, Unconjugated (bsm-51374M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PGK1 Monoclonal Antibody, Unconjugated (bsm-51374M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PGK1 Monoclonal Antibody, Unconjugated (bsm-51374M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PGK1 Monoclonal Antibody, Unconjugated (bsm-51374M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PGK1 Monoclonal Antibody, Unconjugated (bsm-51374M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
GAPDH was immunoprecipitated from Mouse Cerebrum tissue lysate with bsm-51374M at 1\/50 dilution. Western blot was performed from the immunoprecipitate using protein A\/G beads. HRP conjugated Goat Anti-Mouse IgG (light chain specific) was used as secondary antibody.\r\nLane 1: Mouse Cerebrum tissue lysate (Input).\r\nLane 2: bsm-51374M IP in Mouse Cerebrum tissue lysate.\r\nLane 3: native mouse IgG IP in Mouse Cerebrum tissue lysate (negative control).\r\n
GAPDH was immunoprecipitated from Mouse Cerebrum tissue lysate with bsm-51374M at 1\/50 dilution. Western blot was performed from the immunoprecipitate using protein A\/G beads. HRP conjugated Goat Anti-Mouse IgG (light chain specific) was used as secondary antibody.\r\nLane 1: Mouse Cerebrum tissue lysate (Input).\r\nLane 2: bsm-51374M IP in Mouse Cerebrum tissue lysate.\r\nLane 3: native mouse IgG IP in Mouse Cerebrum tissue lysate (negative control).\r\n
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-F
  • IF(IHC-P)
  • IHC-P

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-51374m-100uL
Product Name PGK1 Monoclonal Antibody
Applications
WB IHC-F IF(IHC-P) IHC-P
Reactivity Human, Mouse
Specifications
Conjugation Unconjugated
Host Mouse
Source This antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-417 amino acids from human.
Immunogen Range 1-417/417
Clonality Monoclonal
Clone # 6C1
Isotype IgG2a
Concentration Lot Dependent
Purification Purified by Protein G.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 5230
Swiss Prot P00558
Synonyms PGKA; MIG10; HEL-S-68p; Phosphoglycerate kinase 1; Cell migration-inducing gene 10 protein; Primer recognition protein 2; PRP 2; PGK1; OK/SW-cl.110
Background In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed:2324090). May play a role in sperm motility (PubMed:26677959).
Application Dilution
WB 1:500-2000
IHC-F IHC-F1:100-500
IF(IHC-P) IFIHC-P1:100-500
IHC-P IHC-P1:100-500

Frequently asked questions

Which applications is PGK1 Monoclonal Antibody (bsm-51374M) validated for, and at what dilution?

bsm-51374M is validated for WB(WB) (1:500-2000); IHC-F(IHC-F) (1:100-500); IF(IHC-P)(IF(IHC-P)) (1:100-500); IHC-P(IHC-P) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PGK1 Monoclonal Antibody (bsm-51374M) react with?

Tested reactivity: Human, Mouse.

How should bsm-51374M be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PGK1 Monoclonal Antibody (bsm-51374M)?

This antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-417 amino acids from human. Immunogen range: 1-417/417.

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