RAB20 (4C4) Monoclonal Antibody

$Lane 1: HeLa Cell lysates; Lane 2: MCF-7 Cell lysates; probed with RAB20 (4C4) Monoclonal Antibody, unconjugated (bsm-51377M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: HeLa Cell lysates; Lane 2: MCF-7 Cell lysates; probed with RAB20 (4C4) Monoclonal Antibody, unconjugated (bsm-51377M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Hela cells probed with using RAB20 (4C4) Monoclonal Antibody bsm-51377M at 1:25 for 60 minutes followed by A488 secondary antibody (green) at 1:200 compared to an isotype control of mouse IgG2a (blue).

Applications

Predicted Reactivity

Human

Overview
Catalog #	bsm-51377M
Product Name	RAB20 (4C4) Monoclonal Antibody
Applications	WB, FCM
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This RAB20 antibody is generated from a mouse immunized with recombinant protein from human RAB20.
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG2a
Concentration	0.5ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	55647
Swiss Prot	Q9NX57
Synonyms	Ras-related protein Rab-20; RAB20
Background	Plays a role in apical endocytosis/recycling. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes.
Application Dilution
WB	1:300-5000
FCM	1:20-100