PSMA5 (6T1) Monoclonal Antibody

$Lane 1: Human Liver Lysates; Lane 2: MCF-7 Cell Lysates; Lane 3: HepG2 Cell Lysates; Lane 4: HeLa Cell Lysates; Lane 5: PC-12 Cell Lysates; probed with PSMA5 (426CT8.5.1) Monoclonal Antibody, unconjugated (bsm-51389M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Human Liver Lysates; Lane 2: MCF-7 Cell Lysates; Lane 3: HepG2 Cell Lysates; Lane 4: HeLa Cell Lysates; Lane 5: PC-12 Cell Lysates; probed with PSMA5 (426CT8.5.1) Monoclonal Antibody, unconjugated (bsm-51389M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed, paraffin embedded human testis tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PSMA5 (426CT8.5.1) Monoclonal Antibody (bsm-51389M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human testis tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PSMA5 (426CT8.5.1) Monoclonal Antibody (bsm-51389M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Applications

WB
IHC-P
IF

Reactivity

Human
Rat

Overview
Catalog #	bsm-51389M
Product Name	PSMA5 (6T1) Monoclonal Antibody
Applications	WB, IHC-P, IF
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Purified His-tagged PSMA5 protein(Fragment) was used to produced this monoclonal antibody.
Clonality	Monoclonal
Clone #	6T1
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	5686
Swiss Prot	P28066
Synonyms	PSC5; ZETA; Proteasome subunit alpha type-5; Macropain zeta chain; Multicatalytic endopeptidase complex zeta chain; Proteasome zeta chain; PSMA5
Background	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF

Applications

Reactivity

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