YY1 (3C6) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51412M-100uL |
| Product Name | YY1 (3C6) Monoclonal Antibody |
| Applications |
WB
IHC-P
FCM
|
| Reactivity | Human, Rat, Monkey |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Purified His-tagged YY1 protein was used to produced this monoclonal antibody. |
| Clonality | Monoclonal |
| Clone # | 3C6 |
| Isotype | IgG2a |
| Concentration | Lot Dependent |
| Purification | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purifie |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 7528 |
| Swiss Prot | P25490 |
| Synonyms | DELTA; NF-E1; UCRBP; INO80S; YIN-YANG-1; Transcriptional repressor protein YY1; Delta transcription factor; INO80 complex subunit S; Yin and yang 1; YY-1; YY1 |
| Background | Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (PubMed:15329343). Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions). Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; proposed to target the INO80 complex to YY1-responsive elements. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is YY1 (3C6) Monoclonal Antibody (bsm-51412M) validated for, and at what dilution?
bsm-51412M is validated for WB (1:300-5000); IHC-P (1:200-400); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does YY1 (3C6) Monoclonal Antibody (bsm-51412M) react with?
Tested reactivity: Human, Rat, Monkey.
How should bsm-51412M be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise YY1 (3C6) Monoclonal Antibody (bsm-51412M)?
Purified His-tagged YY1 protein was used to produced this monoclonal antibody.