MAGED1 (5C7) Monoclonal Antibody

$Lane 1: A2058; Lane 2: A375; Lane 3: Jurkat cell lysate at 20 \u00b5g per lane, probed with bsm-51428M MAGED1 (5C7) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.$

Lane 1: A2058; Lane 2: A375; Lane 3: Jurkat cell lysate at 20 \u00b5g per lane, probed with bsm-51428M MAGED1 (5C7) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51428M
Product Name	MAGED1 (5C7) Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This MAGED1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 20-224 amino acids from the Central region of human MAGED1.
Clonality	Monoclonal
Clone #	5C7
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	9500
Swiss Prot	Q9Y5V3
Synonyms	NRAGE; DLXIN-1; Melanoma-associated antigen D1; MAGE tumor antigen CCF; MAGE-D1 antigen; Neurotrophin receptor-interacting MAGE homolog; MAGED1; PP2250; PRO2292
Background	Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Inhibits cell cycle progression, and facilitates NGFR-mediated apoptosis. May act as a regulator of the function of DLX family members. May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Plays a role in the circadian rythm regulation. May act as RORA co-regulator, modulating the expression of core clock genes such as ARNTL/BMAL1 and NFIL3, induced, or NR1D1, repressed.
Application Dilution
WB	1:300-5000